ʻO Tris Hydrochloride / TRIS-HCL kahi paʻa paʻa i nā noi biological e like me electrochromatography, UV analysis a me HPLC.Hoʻohana ʻia e hoʻoponopono a hoʻopaʻa i nā pae pH no nā gel i hoʻohana ʻia i nā noi electrophoresis.Hoʻohana nui ʻia ʻo Tris Hydrochloride ma ke ʻano he buffer biological a i ʻole he ʻāpana o nā hopena buffer.
Tris Hydrochloride/TRIS-HCL
CAS: 1185-53-1
MF: C4H12ClNO3
MW: 157.6
EINECS: 214-684-5
Ka helu heheʻe 150-152 °C
mānoanoa 1.05 g/mL ma 20 °C
ka mahana mālama.Hale kūʻai ma RT.
solubility H2O: 4 M ma 20 °C, akaka, kala ole
ʻano crystalline
kala ʻaʻole waihoʻoluʻu ʻole (40 % (w/w) i ka hāʻina H2O).
Tris Hydrochloride/TRIS-HCL CAS 1185-53-1
Nā mea | Hōʻike | Nā hopena hoʻāʻo |
Ka nana aku | ʻO ka pauka kristal keʻokeʻo | Kūlike |
Solubility (1M aq.) | ʻO ka hāʻina akaka, kala ʻole | Kūlike |
Nā metala kaumaha | ≤5ppm | Kūlike |
pH (1% aq.) | 4.2~5.0 | 4.4 |
Hoʻāʻo | 99.0%~101.0% | 100.5% |
Ka hoʻopaʻa ʻana o UV/260nm (1M aq.) | ≤0.06% | 0.012% |
Ka hoʻopaʻa ʻana o UV/280nm (1M aq.) | ≤0.05% | 0.02% |
Waihona | Ka wela lumi |
Hoʻohana mau ʻia ʻo Tris Hydrochloride/TRIS-HCL CAS 1185-53-1 ma ke ʻano he ʻāpana pale o ka phenol extraction o DNA a i ʻole RNA a me kahi mea hoʻopaʻa i ka hoʻokaʻawale a hoʻopaʻa ʻana i nā gels i ke ʻano o nā huahana protein e SDS-PAGE.Ua hoʻohana pū ʻia ʻo Tris Hydrochloride me ka urea ma ke ʻano he ala no ka hoʻihoʻi antigen i ka immunohistochemistry.I loko o nā ʻiʻo maʻemaʻe, ua ʻike ʻia ʻo Tris Hydrochloride e kaohi i ka pane ʻana o nā kaʻa i ka hoʻoulu ʻana i ke aʻalolo adrenergic motor.I ka crystalization o ka porcine pancreatic elastase me ka hoʻohana ʻana iā Tris Hydrochloride, ua ʻike ʻia ka Tris Hydrochloride buffer e hoʻohuli i ka hoʻololi conformational a me ka hoʻopaʻa ʻana i ka crystal-packing.Ua hoʻokomo pū ʻia ʻo Tris Hydrochloride i loko o ka wai coelomic a ma Cortland medium e mālama ai i nā hua iʻa ʻānuenue i hoʻomoʻa ʻole ʻia.ʻIke ʻia ʻo Tris Hydrochloride ʻo Tris(hydroxymethyl)aminomethane hydrochloride a me Tris HCl.
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